ADENOSINE TRIPHOSPHATE CONTENT OF PREIMPLANTATION MOUSE EMBRYOS
نویسندگان
چکیده
منابع مشابه
Adenosine triphosphate content of preimplantation mouse embryos.
The utilization of energy substrates by preimplantation mouse embryos undergoes several changes during development in vitro (Brinster, 1965a, b) but very little is known about the level of high-energy containing compounds, such as ATP at these stages. In the present study, the content ofATP in freshly collected preimplantation mouse embryos has been measured. For each estimation, fifty to 150 e...
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Background: The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. Methods: The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...
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Two-cell mouse embryos were cultured in vitro for different periods in a medium in which NaCl was partially replaced by LiCl at concentrations ranging from 1 to 30 mM. The relative cell number diminished according to increasing LiCl concentrations but the onset of blastulation was not affected, thus resulting in blastulae with fewer cells than normal and with a reduced or absent inner cell mass...
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Diploid gynogenetic mouse embryos were produced in a three-step procedure: fertilization in vitro, suppression of the 2nd polar body formation by Cytochalasin B, and microsurgical removal of the male pronucleus. The operated eggs were transplanted to the oviduct of recipient females for 72 or 96 h. The overall recovery rate was 73%, but compacted morulae and blastocysts constituted only 28.6% o...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1971
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0250133